Model 2200 Dual GC - Multidimensional Chiral
Analysis System
Introduction
The advent of Cydex™ based chiral columns added
another dimension to versatility of chromatographic separation. The ability to
separate positional isomers was a key importance to laboratories involved in
natural products, such as flavors and fragrances. Chiral columns, however, offer
several challenges for the analytical chemist.
These include:
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Upper temperature limits
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Very slow oven ramp rates
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Small-bore, low flow column, with limited sample capacity
To address these problems, Microanalytics developed
the Model 2200, which gives the natural products or pharmaceutical
chemist a new level of separation and characterization capabilities.
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Model 2200 Approach
These abovementioned factors make it difficult to analyze a typically complex
flavor or fragrance on a standard single chiral column GC. A multidimensional approach
with dual ovens offers a number of advantages. A example system would have a
precolumn in one oven, with one or more chiral columns in the second oven.
The 2nd oven allows the user to precisely control the optimal separation
temperature and ramp rate for the chiral column(s).
By
connecting two(2), 320µm ID columns in parallel, we can approximate the flow
characteristics of a single 530µm ID column. This allows the coupling of the chiral
columns to a high capacity, "megabore" column. Also, as one chiral phase does
not separate all of the possible isomers, we can couple 2 different phases for
simultaneous analysis with a single injection, a b-Cydex
and a g-Cydex, for example.
The heartcut mechanism at the column juncture allows single or
multiple heartcuts of, in some cases, a single peak or portion thereof. This single compound is
then routed into the two chiral columns for separation. By heart-cutting a single
peak, or only a portion of a single peak, you reduce the complexity of the
chiral separation dramatically. Also, the inclusion of a mid-point split
assembly allows the user to precisely load the two chiral columns with the
optimal amount of sample.
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All of the above refinements are included in the Model
2200 Chiral Analysis system.
As this system is typically custom designed for each customer's
special needs, so please contact Microanalytics for
a custom design and quotation for this system.
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